Chromatin Immunoprecipitation:Article Title: Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages
Article Snippet: .. 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Ly6G (141 Pr) Fluidigm Cat# 3141008B; RRID: AB_2814678 CD11c (142 Nd) Fluidigm Cat# 3142003B; RRID: AB_2814737 CD8a (168Er) Fluidigm Cat# 3168003B; RRID: AB_2811241 CD11b (172Yb) Fluidigm Cat# 3172012B; RRID: AB_2661809 CD19 (149 Sm) Fluidigm Cat# 3149002B; RRID: AB_2814679 CD25 (151 Eu) Fluidigm Cat# 3151007B; RRID: AB_2827880 F4/80 (146 Nd) Fluidigm Cat# 3146008B; RRID: AB_2895117 CD36 (147 Sm) Fluidigm Cat# 3147018B;RRID:N/A CD3e (152 Sm) Fluidigm Cat# 3152004B; RRID: AB_2687836 CD274 (153Eu) Fluidigm Cat# 3153016B; RRID: AB_2687837 CD62L (160 Gd) Fluidigm Cat# 3160008B; RRID: AB_2687840 CD73 (154 Sm) Fluidigm Cat# 3154019B; RRID: AB_2813854 Ly6c (150Nd) Fluidigm Cat# 3150010B; RRID: AB_2895118 CX3CR1 (164 Dy) Fluidigm Cat# 3164023B; RRID: AB_2832247 CD103 (163 Dy) This study N/A CD206 (169 Tm) Fluidigm Cat# 3169021B; RRID: AB_2832249 NK1.1 (170 Er) Fluidigm Cat# 3170002B; RRID: AB_2885023 CD44 (162Dy) Fluidigm Cat# 3162030B; RRID: AB_2814898 CD4 (145Nd) Fluidigm Cat# 3145002B; RRID: AB_2687832 MHCII (209Bi) Fluidigm Cat# 3209006B; RRID: AB_2885025 CD45 (89Y) Fluidigm Cat# 3089005B; RRID: AB_2651152 B220 (176 Yb) Fluidigm Cat# 3176002B; RRID: AB_2895123 All flow cytometer antibodies were obtained from Thermo Fisher Scientific except specified. .. PerCP-Cy5.5 labeled anti-IL-17A (TC11-18H10.1, Biolegend), 506943 PE- labeled anti-IL-17A (TC11-18H10.1, Biolegend), 506903 FITC- labeled anti-IL-17A (TC11-18H10.1), A15377 APC-labeled anti-IL-17A (TC11-18H10.1, Biolegend), cat# 506915 PE- labeled anti-IL-4 (11B11), Cat# 12-7101-41; APC-labeled anti-IL-4 (11B11) Cat# 17-7041-82; PE--labelled anti-IL-10 (JES5–16E3) Cat# 12-7101-82 APC-labelled anti-IL-10 (JES5–16E3), Cat# 17-7101-82 APC- labeled anti-IFN-! (XMG1.2), Cat# 17-7311-82 PE-Cy7-labeled anti-IFN-! (XMG1.2), Cat# 25-7311-82 PE-labeled anti-Foxp3 (FJK-16s), Cat# 12-5773-82 PE- labeled anti-CD11b (M1/70), Cat# 12-0112-82 FITC- labeled anti-CD11b (M1/70), Cat# 12-0112-82 APC-labeled anti-CD11b (M1/70), Cat# 17-0112-82 PE- labeled anti-CD4 (RM4-5), Cat# 12-0042-82 FITC- labeled anti-CD4 (RM4-5), Cat# 12-0042-82 APC-labeled anti-CD4 (RM4-5), Cat# 17-0042-82 PE-Cy7-labeled anti-CD3 (145-2C11), Cat# 25-0031-82 PE-anti-Gr-1 (RB6-8C5), Cat# 12-5931-82 PE- labeled anti-mouse Ly6G (1A8), Cat# 12-9668-82 FITC-labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 APC-conjugated CD45.2 (104), Cat# 17-0454-82 PE-conjugated anti-CD45.1 (A20), Cat# 12-0453-82 FITC- labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 PerCP-Cy5.5 labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 APC-anti-Lag-3 (C9B7W), Cat# 17-2231-82 PE-anti-CD244.2 (2B4), Cat# 12-2441-82 PE-anti-TOX (TXRX10), Cat# 12-6502-82 PE-anti-CD39 (24DM1), Cat# 25-0391-82 PE-anti-Trem2 (237920, R&D), Cat# FAB17291P 4 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript.
Flow Cytometry:Article Title: Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages
Article Snippet: .. 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Ly6G (141 Pr) Fluidigm Cat# 3141008B; RRID: AB_2814678 CD11c (142 Nd) Fluidigm Cat# 3142003B; RRID: AB_2814737 CD8a (168Er) Fluidigm Cat# 3168003B; RRID: AB_2811241 CD11b (172Yb) Fluidigm Cat# 3172012B; RRID: AB_2661809 CD19 (149 Sm) Fluidigm Cat# 3149002B; RRID: AB_2814679 CD25 (151 Eu) Fluidigm Cat# 3151007B; RRID: AB_2827880 F4/80 (146 Nd) Fluidigm Cat# 3146008B; RRID: AB_2895117 CD36 (147 Sm) Fluidigm Cat# 3147018B;RRID:N/A CD3e (152 Sm) Fluidigm Cat# 3152004B; RRID: AB_2687836 CD274 (153Eu) Fluidigm Cat# 3153016B; RRID: AB_2687837 CD62L (160 Gd) Fluidigm Cat# 3160008B; RRID: AB_2687840 CD73 (154 Sm) Fluidigm Cat# 3154019B; RRID: AB_2813854 Ly6c (150Nd) Fluidigm Cat# 3150010B; RRID: AB_2895118 CX3CR1 (164 Dy) Fluidigm Cat# 3164023B; RRID: AB_2832247 CD103 (163 Dy) This study N/A CD206 (169 Tm) Fluidigm Cat# 3169021B; RRID: AB_2832249 NK1.1 (170 Er) Fluidigm Cat# 3170002B; RRID: AB_2885023 CD44 (162Dy) Fluidigm Cat# 3162030B; RRID: AB_2814898 CD4 (145Nd) Fluidigm Cat# 3145002B; RRID: AB_2687832 MHCII (209Bi) Fluidigm Cat# 3209006B; RRID: AB_2885025 CD45 (89Y) Fluidigm Cat# 3089005B; RRID: AB_2651152 B220 (176 Yb) Fluidigm Cat# 3176002B; RRID: AB_2895123 All flow cytometer antibodies were obtained from Thermo Fisher Scientific except specified. .. PerCP-Cy5.5 labeled anti-IL-17A (TC11-18H10.1, Biolegend), 506943 PE- labeled anti-IL-17A (TC11-18H10.1, Biolegend), 506903 FITC- labeled anti-IL-17A (TC11-18H10.1), A15377 APC-labeled anti-IL-17A (TC11-18H10.1, Biolegend), cat# 506915 PE- labeled anti-IL-4 (11B11), Cat# 12-7101-41; APC-labeled anti-IL-4 (11B11) Cat# 17-7041-82; PE--labelled anti-IL-10 (JES5–16E3) Cat# 12-7101-82 APC-labelled anti-IL-10 (JES5–16E3), Cat# 17-7101-82 APC- labeled anti-IFN-! (XMG1.2), Cat# 17-7311-82 PE-Cy7-labeled anti-IFN-! (XMG1.2), Cat# 25-7311-82 PE-labeled anti-Foxp3 (FJK-16s), Cat# 12-5773-82 PE- labeled anti-CD11b (M1/70), Cat# 12-0112-82 FITC- labeled anti-CD11b (M1/70), Cat# 12-0112-82 APC-labeled anti-CD11b (M1/70), Cat# 17-0112-82 PE- labeled anti-CD4 (RM4-5), Cat# 12-0042-82 FITC- labeled anti-CD4 (RM4-5), Cat# 12-0042-82 APC-labeled anti-CD4 (RM4-5), Cat# 17-0042-82 PE-Cy7-labeled anti-CD3 (145-2C11), Cat# 25-0031-82 PE-anti-Gr-1 (RB6-8C5), Cat# 12-5931-82 PE- labeled anti-mouse Ly6G (1A8), Cat# 12-9668-82 FITC-labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 APC-conjugated CD45.2 (104), Cat# 17-0454-82 PE-conjugated anti-CD45.1 (A20), Cat# 12-0453-82 FITC- labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 PerCP-Cy5.5 labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 APC-anti-Lag-3 (C9B7W), Cat# 17-2231-82 PE-anti-CD244.2 (2B4), Cat# 12-2441-82 PE-anti-TOX (TXRX10), Cat# 12-6502-82 PE-anti-CD39 (24DM1), Cat# 25-0391-82 PE-anti-Trem2 (237920, R&D), Cat# FAB17291P 4 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript.
Magnetic Resonance Imaging:Article Title: Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages
Article Snippet: .. 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Ly6G (141 Pr) Fluidigm Cat# 3141008B; RRID: AB_2814678 CD11c (142 Nd) Fluidigm Cat# 3142003B; RRID: AB_2814737 CD8a (168Er) Fluidigm Cat# 3168003B; RRID: AB_2811241 CD11b (172Yb) Fluidigm Cat# 3172012B; RRID: AB_2661809 CD19 (149 Sm) Fluidigm Cat# 3149002B; RRID: AB_2814679 CD25 (151 Eu) Fluidigm Cat# 3151007B; RRID: AB_2827880 F4/80 (146 Nd) Fluidigm Cat# 3146008B; RRID: AB_2895117 CD36 (147 Sm) Fluidigm Cat# 3147018B;RRID:N/A CD3e (152 Sm) Fluidigm Cat# 3152004B; RRID: AB_2687836 CD274 (153Eu) Fluidigm Cat# 3153016B; RRID: AB_2687837 CD62L (160 Gd) Fluidigm Cat# 3160008B; RRID: AB_2687840 CD73 (154 Sm) Fluidigm Cat# 3154019B; RRID: AB_2813854 Ly6c (150Nd) Fluidigm Cat# 3150010B; RRID: AB_2895118 CX3CR1 (164 Dy) Fluidigm Cat# 3164023B; RRID: AB_2832247 CD103 (163 Dy) This study N/A CD206 (169 Tm) Fluidigm Cat# 3169021B; RRID: AB_2832249 NK1.1 (170 Er) Fluidigm Cat# 3170002B; RRID: AB_2885023 CD44 (162Dy) Fluidigm Cat# 3162030B; RRID: AB_2814898 CD4 (145Nd) Fluidigm Cat# 3145002B; RRID: AB_2687832 MHCII (209Bi) Fluidigm Cat# 3209006B; RRID: AB_2885025 CD45 (89Y) Fluidigm Cat# 3089005B; RRID: AB_2651152 B220 (176 Yb) Fluidigm Cat# 3176002B; RRID: AB_2895123 All flow cytometer antibodies were obtained from Thermo Fisher Scientific except specified. .. PerCP-Cy5.5 labeled anti-IL-17A (TC11-18H10.1, Biolegend), 506943 PE- labeled anti-IL-17A (TC11-18H10.1, Biolegend), 506903 FITC- labeled anti-IL-17A (TC11-18H10.1), A15377 APC-labeled anti-IL-17A (TC11-18H10.1, Biolegend), cat# 506915 PE- labeled anti-IL-4 (11B11), Cat# 12-7101-41; APC-labeled anti-IL-4 (11B11) Cat# 17-7041-82; PE--labelled anti-IL-10 (JES5–16E3) Cat# 12-7101-82 APC-labelled anti-IL-10 (JES5–16E3), Cat# 17-7101-82 APC- labeled anti-IFN-! (XMG1.2), Cat# 17-7311-82 PE-Cy7-labeled anti-IFN-! (XMG1.2), Cat# 25-7311-82 PE-labeled anti-Foxp3 (FJK-16s), Cat# 12-5773-82 PE- labeled anti-CD11b (M1/70), Cat# 12-0112-82 FITC- labeled anti-CD11b (M1/70), Cat# 12-0112-82 APC-labeled anti-CD11b (M1/70), Cat# 17-0112-82 PE- labeled anti-CD4 (RM4-5), Cat# 12-0042-82 FITC- labeled anti-CD4 (RM4-5), Cat# 12-0042-82 APC-labeled anti-CD4 (RM4-5), Cat# 17-0042-82 PE-Cy7-labeled anti-CD3 (145-2C11), Cat# 25-0031-82 PE-anti-Gr-1 (RB6-8C5), Cat# 12-5931-82 PE- labeled anti-mouse Ly6G (1A8), Cat# 12-9668-82 FITC-labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 APC-conjugated CD45.2 (104), Cat# 17-0454-82 PE-conjugated anti-CD45.1 (A20), Cat# 12-0453-82 FITC- labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 PerCP-Cy5.5 labeled anti-mouse Ly6G (1A8), Cat# 11-9668-82 APC-anti-Lag-3 (C9B7W), Cat# 17-2231-82 PE-anti-CD244.2 (2B4), Cat# 12-2441-82 PE-anti-TOX (TXRX10), Cat# 12-6502-82 PE-anti-CD39 (24DM1), Cat# 25-0391-82 PE-anti-Trem2 (237920, R&D), Cat# FAB17291P 4 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript.
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